Cell Migration on Suspended Fibers
Cell migration is a fundamental process necessary for the creation of tissues during embryogenesis, immune surveillance, wound repair, inflammation, and the invasion and metastasis of cancer cells. All of these processes occur in the context of the extracellular matrix. For decades, there has been a concerted effort to understand the basic mechanisms of cell migration and other cellular behaviors, focused largely on cells cultured on 2D surfaces. Studies in vivo demonstrate that cells also use migratory approaches different than those observed on 2D surfaces in vitro, and even 2D migration in vivo occurs in the context of 3D tissue. Recently, there has been a move to apply the wealth of knowledge regarding what we know about 2D cell migration to understand cell migration within the context of 3D matrices and in vivo. Leaders in the field have identified several challenges to this task, which include the fact that migration in 3D matrices in vivo is quite different from 2D matrices, the difficulty creating relevant in vitro matrices that capture matrix composition and topography of in vivo microenvironments, and the challenges of manipulating the environment in vivo”.
- Patti Keely (2015)
Our Approach
At STEP Lab, we deconstruct the effects of the various physical characteristics of the ECM on single and collective cell migration in precisely controlled environments. Through design of suspended fiber networks of varying attributes, we have demonstrated the following migratory responses: